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培養歯肉上皮細胞の細胞運動におよぼす低出力レーザーの影響
https://ohu-lib.repo.nii.ac.jp/records/2152
https://ohu-lib.repo.nii.ac.jp/records/2152751869f5-b6b3-4c29-b2b6-994818e30362
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
KJ00000731675.pdf (1.6 MB)
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| Item type | 紀要論文(ELS)_JAIRO Cloud（WEKO3）対応_596d4a9f(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2003-06-30 | |||||
| タイトル | ||||||
| タイトル | 培養歯肉上皮細胞の細胞運動におよぼす低出力レーザーの影響 | |||||
| タイトル | ||||||
| タイトル | Low-power Laser Accelerates Cell Motility of Gingival Keratinocytes in vitro | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | low-power laser | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | in vitro | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | cell motility | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Cdc42-Rac | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | gingival keratinocyte | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| ページ属性 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | P(論文) | |||||
| 記事種別(日) | ||||||
| 値 | 原著論文 | |||||
| 記事種別(英) | ||||||
| 言語 | en | |||||
| 値 | Original Article | |||||
| 著者名(日) |
土肥, 宏樹
× 土肥, 宏樹× 齋藤, 勇× 西上, 堅二× 月田, 秀夫× 田中, 三千三郎× 安部, 仁晴× 中川, 敏浩× 山本, 茂久 |
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| 著者名よみ |
ドヒ, ヒロキ
× ドヒ, ヒロキ× サイトウ, イサム× ニシガミ, ケンジ× ツキダ, ヒデオ× タナカ, ミチサブロウ× アベ, キミハル× ナカガワ, トシヒロ× ヤマモト, シゲヒサ |
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| 著者名(英) |
DOHI, Hiroki
× DOHI, Hiroki× SAITO, Isamu× NISHIGAMI, Kenji× TSUKIDA, Hideo× TANAKA, Michisaburou× AMBE, Kimiharu× NAKAGAWA, Toshihiro× YAMAMOTO, Shigehisa |
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| 著者所属(日) | ||||||
| 値 | 奥羽大学歯学部口腔解剖学第II講座 | |||||
| 著者所属(日) | ||||||
| 値 | 奥羽大学歯学部口腔細菌学講座 | |||||
| 著者所属(日) | ||||||
| 値 | 奥羽大学歯学部口腔解剖学第II講座 | |||||
| 著者所属(日) | ||||||
| 値 | 奥羽大学歯学部口腔解剖学第II講座 | |||||
| 著者所属(日) | ||||||
| 値 | 奥羽大学歯学部口腔解剖学第II講座 | |||||
| 著者所属(日) | ||||||
| 値 | 奥羽大学歯学部口腔解剖学第II講座 | |||||
| 著者所属(日) | ||||||
| 値 | 奥羽大学歯学部口腔解剖学第II講座 | |||||
| 著者所属(日) | ||||||
| 値 | 奥羽大学歯学部口腔解剖学第II講座 | |||||
| 著者所属(英) | ||||||
| 言語 | en | |||||
| 値 | Second Department of Oral Anatomy, Ohu University School of Dentistry | |||||
| 著者所属(英) | ||||||
| 言語 | en | |||||
| 値 | Department of Oral Bacteriology, Ohu University School of Dentistry | |||||
| 著者所属(英) | ||||||
| 言語 | en | |||||
| 値 | Second Department of Oral Anatomy, Ohu University School of Dentistry | |||||
| 著者所属(英) | ||||||
| 言語 | en | |||||
| 値 | Second Department of Oral Anatomy, Ohu University School of Dentistry | |||||
| 著者所属(英) | ||||||
| 言語 | en | |||||
| 値 | Second Department of Oral Anatomy, Ohu University School of Dentistry | |||||
| 著者所属(英) | ||||||
| 言語 | en | |||||
| 値 | Second Department of Oral Anatomy, Ohu University School of Dentistry | |||||
| 著者所属(英) | ||||||
| 言語 | en | |||||
| 値 | Second Department of Oral Anatomy, Ohu University School of Dentistry | |||||
| 著者所属(英) | ||||||
| 言語 | en | |||||
| 値 | Second Department of Oral Anatomy, Ohu University School of Dentistry | |||||
| 抄録(英) | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Low-power laser irradiation on oral mucosae is supposed to have healing effects caused by accelerating cell growth. However, the mechanism of epithelial cell growth caused by laser irradiation is unknown. The aim of this study is to solve effects of low-power laser irradiation on epithelial cell motility which is an important factor in wound healing. Experimental cells were normal human gingival keratinocytes, cultured in keratinocyte growth medium-2 for 2 days. Low-power laser device was trinpl D^[○!R] (905nm, GaAlAs diode, average 2.4mW, YOSHIDA, Tokyo). The cell motility of gingival keratinocytes in vitro were examined by phase contrast microscopy with CCD camera, and the serials image of the same cell photographed every 10 minutes for 360 minutes were analyzed by image analysis software program. Cdc42/Rac which is a member of the Rho family in gingival keratinocytes was examined by confocal laser scanning microscopy using antibody. Cells in non-irradiation group moved toward their forming filopodia and lamellipodia at average speed of 55μm/hr through examination period. In irradiation group, the speed of cell movement increased after laser irradiation, and reached a peak of 90μm/hrin 30 minutes. Afterwards, the speed reduced and was almost the same as non-irradiation group in 300 minutes later. On the other hand, Cdc42/Rac in non-irradiation group was expressed on a part of lamellipodia region of the cell. In irradiation group, expression of Cdc42/Rac was more than in non-irradiation group. From these results, low-power laser irradiation appeared to accelerate cell motility of gingival keratinocyte. Further, low-power laser may influence signal transduction of Rho family related to cell motility in wound healing. | |||||
| 雑誌書誌ID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AN1010214X | |||||
| 書誌情報 |
奥羽大学歯学誌 巻 30, 号 2, p. 141-147, 発行日 2003-06-30 |
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