@article{oai:ohu-lib.repo.nii.ac.jp:00002160,
 author = {馬場, 園子 and ババ, ソノコ and BABA, Sonoko},
 issue = {2},
 journal = {奥羽大学歯学誌},
 month = {Jun},
 note = {P(論文), To find out salivary proteins related to bitter taste, quinine sulfate showing fluorescence under UV radiation was utilized. Resting human saliva was collected from normal and abnormal subjects for quinine sulfate threshold. The salivary proteins added 0.08% quinine sulfate solution were separated on 2% agarose gel electrophoresis. After detecting the fluorescent band under UV radiation, it was separated with 18.0% SDS-PAGE and then the proteins were transferred to PVDF membrane to elute. These were analyzed with an amino acid sequencer. Comparing the peptides of normal subjects with that of abnormal subjects in SDS-PAGE, a band less than 6.5kDa containing quinine sulfate was recognized on normal subjects. One well-defined band identified with CBB staining of the agarose gel corresponds to the fluorescent portion of the gel, suggesting the quinine might bind to a certain peptide in saliva. Two peptides, Histatin 3, 5, or 6 and basic Proline-Rich Peptide P-E, were detected with partial sequence analysis. These two are candidates as bitter-binding peptides, although their functions have not yet been identified.},
 pages = {189--196},
 title = {苦味に関連する唾液中タンパク質の検出},
 volume = {30},
 year = {2003}
}