@article{oai:ohu-lib.repo.nii.ac.jp:00002209, author = {茂呂, 祐利子 and 中川, 敏浩 and 安部, 仁晴 and 齋藤, 勇 and 山本, 茂久 and モロ, ユリコ and ナカガワ, トシヒロ and アベ, キミハル and サイトウ, イサム and ヤマモト, シゲヒサ and MORO, Yuriko and NAKAGAWA, Toshihiro and AMBE, Kimiharu and SAITO, Isamu and YAMAMOTO, Shigehisa}, issue = {4}, journal = {奥羽大学歯学誌}, month = {Dec}, note = {P(論文), Low-power laser irradiation on oral tissue is reported to have healing effects caused by accelerating cell growth. However, the effects of high-power laser irradiation on cell growth are unknown. To investigate the effects of high-power laser irradiation on oral tissue, we examined the cell growth of fibroblasts (Balb/3T3(clone)A31), osteoblasts (MC3T3-E1) and epithelial cells (normal human gingival keratinocytes) after laser irradiation in vitro using MTT assay. Cells were cultured during 1 day following laser irradiation once for 30, 60, 90, 120 and 180 seconds with a diode laser (830±10nm, GaAlAs diode, continuous wave, 400mW or 800mW). Non-irradiation cells were used as the control group. As a result, fibroblasts and epithelial cells showed good cell growth under the irradiation of 3.7mW/cm^2 for 90seconds. The irradiation of 3.7mW/cm^2 for 90seconds and of 6.7mW/cm^2 for 60seconds accelerated the cell growth of osteoblasts. These results suggest that laser irradiation of 3.7mW/cm^2 for 90seconds accelerates cell growth. In addition, power density (mW/cm^2) and irradiation time (seconds) are more closely related to cell growth in vitro than to energy density (J/cm^2).}, pages = {271--279}, title = {培養細胞の増殖におよぼすレーザーの出力とエネルギー密度に関する研究}, volume = {30}, year = {2003} }