@article{oai:ohu-lib.repo.nii.ac.jp:00002232,
 author = {千葉, 有 and 釜田, 朗 and 松渕, 志帆 and 田谷, かほる and 齋藤, 高弘 and 五十嵐, 治義 and チバ, ユウ and カマダ, アキラ and マツブチ, シホ and タヤ, カホル and サイトウ, タカヒロ and イガラシ, ハルヨシ and CHIBA, Yu and KAMADA, Akira and MATSUBUCHI, Shiho and TAYA, Kahoru and SAITO, Takahiro and IGARASHI, Haruyoshi},
 issue = {1},
 journal = {奥羽大学歯学誌},
 month = {Mar},
 note = {P(論文), Chitosan is a natural macromolecule that can be obtained by deacetylation of chitin in concentrated alkaline solution, and it has been known to exhibit various pharmacological actions, including facilitative effects on wound healing. Chitooligosaccharides are oligosaccharides composed of two to seven glucosamine residues, which are the basic molecules of chitosan. Systemic administration of chitooligosaccharides facilitates wound healing, but its mechanism remains largely unknown. The present study was investigated the effects of systemic administration of chitooligosaccharides on healing of the oral mucous membrane in vitro and in vivo. Human gingiva-derived fibroblast were incubated with 0.25-1.0% of chitooligosaccharides, and the cell number was counted after 24, 48, 72, 96, 120 and 144 hours. In addition, to determine whether systemic administration of chitooligosaccharides facilitates healing of mouse oral mucous membrane damaged by neodymium YAG laser irradiation, the oral mucous membrane was sampled at certain times after laser irradiation and was analyzed histologically. The results were as follows: 1. With 0.5-0.75% of chitooligosaccharides, the number of fibroblasts increased during the early stages of incubation, but with high concentrations of chitooligosaccharides, the number of fibroblast decreased. 2. Systemic administration of chitooligosaccharides facilitated proliferation of fibroblast, while continuous high-dose administration suppressed proliferation. The above findings clarify that chitooligosaccharides facilitate the proliferation of fibroblast in vitro and that systemic administration facilitates wound healing in vivo. In addition, there appear to be optimal doses in both in vitro and in vivo applications.},
 pages = {23--32},
 title = {キトサンオリゴ糖の創傷治癒促進作用},
 volume = {31},
 year = {2004}
}