{"created":"2023-05-15T14:19:08.100125+00:00","id":2645,"links":{},"metadata":{"_buckets":{"deposit":"b0fc0d72-3361-4ea3-bf13-70598c5be4d0"},"_deposit":{"created_by":3,"id":"2645","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"2645"},"status":"published"},"_oai":{"id":"oai:ohu-lib.repo.nii.ac.jp:00002645","sets":["90:111:113"]},"author_link":["9869","9870","9871"],"item_1_biblio_info_14":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2007-06","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"58","bibliographicPageStart":"51","bibliographicVolumeNumber":"34","bibliographic_titles":[{"bibliographic_title":"奥羽大学歯学誌"}]}]},"item_1_creator_6":{"attribute_name":"著者名(日)","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"齋藤, 勇"}],"nameIdentifiers":[{"nameIdentifier":"9869","nameIdentifierScheme":"WEKO"}]}]},"item_1_creator_7":{"attribute_name":"著者名よみ","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"サイトウ, イサム"}],"nameIdentifiers":[{"nameIdentifier":"9870","nameIdentifierScheme":"WEKO"}]}]},"item_1_creator_8":{"attribute_name":"著者名(英)","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"SAITO, Isamu","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"9871","nameIdentifierScheme":"WEKO"}]}]},"item_1_description_1":{"attribute_name":"ページ属性","attribute_value_mlt":[{"subitem_description":"P(論文)","subitem_description_type":"Other"}]},"item_1_description_12":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_description":"Digital images of confocal laser scanning microscope were analyzed by using several macro commands with NIH Image software to reveal the influence of He-Ne laser irradiation on the cytoplasmic organelles in MC3T3-E1 osteogenic cells. The mitochondria were stained by commercial fluorescent probes, named MitoTracker^ Green FM and captured with Nikon PCM2000 confocal laser scanning microscope. Then, these mitochondrial images were analyzed by using macro commands such as stack, center point, summed image, protrusion and retraction. In this paper, it is discussed how exactly to obtain the suitable conditions for processing digital images, such as upper/lower points of a look up table (LUT), range of density slices and smoothing treatment. Furthermore, we recognize that the macro commands with NIH Image software are very useful for analyzing sequential images automatically.","subitem_description_type":"Other"}]},"item_1_source_id_13":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AN1010214X","subitem_source_identifier_type":"NCID"}]},"item_1_text_10":{"attribute_name":"著者所属(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Department of Oral Medical Science Ohu University School of Dentistry"}]},"item_1_text_2":{"attribute_name":"記事種別(日)","attribute_value_mlt":[{"subitem_text_value":"原著論文"}]},"item_1_text_3":{"attribute_name":"記事種別(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Original Article"}]},"item_1_text_9":{"attribute_name":"著者所属(日)","attribute_value_mlt":[{"subitem_text_value":"奥羽大学歯学部口腔病態解析制御学講座"}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2007-06-01"}],"displaytype":"detail","filename":"KJ00004763191.pdf","filesize":[{"value":"1.4 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"url":"https://ohu-lib.repo.nii.ac.jp/record/2645/files/KJ00004763191.pdf"},"version_id":"ef12aeae-87cd-4edb-b988-8f757bc4f8e3"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"NIH image","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"protrusion","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"retraction","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"image analysis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"macro command","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"NIH Imageのマクロによる共焦点レーザー顕微鏡の画像解析","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"NIH Imageのマクロによる共焦点レーザー顕微鏡の画像解析"},{"subitem_title":"Image Analysis of Confocal Laser Scanning Microscope by the Macro Command with NIH Image","subitem_title_language":"en"}]},"item_type_id":"1","owner":"3","path":["113"],"pubdate":{"attribute_name":"公開日","attribute_value":"2007-06-01"},"publish_date":"2007-06-01","publish_status":"0","recid":"2645","relation_version_is_last":true,"title":["NIH Imageのマクロによる共焦点レーザー顕微鏡の画像解析"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-05-15T14:47:57.287064+00:00"}