{"created":"2023-05-15T14:19:52.179428+00:00","id":4044,"links":{},"metadata":{"_buckets":{"deposit":"31efc159-59e4-4c3d-acd2-6bb5079f1f91"},"_deposit":{"created_by":3,"id":"4044","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"4044"},"status":"published"},"_oai":{"id":"oai:ohu-lib.repo.nii.ac.jp:00004044","sets":["90:246:255"]},"author_link":["13532","13533","13535","13534","13531"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014-12","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3-4号","bibliographicPageStart":"123-128","bibliographicVolumeNumber":"41巻","bibliographic_titles":[{"bibliographic_title":"奥羽大学歯学誌"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"CRISPR/Cas9を用いたMC3T3-E1細胞におけるGpr81遺伝子のノックアウトについて検討した。遺伝子導入したMC3T3-E1細胞株(KO)45株のGpr81遺伝子発現量をRT-qPCRで定量しβ-アクチンで補正し、野生型比で72.4%%であった。このうち、約50%の発現を示したものと、50%以下の発現であったものを各1株(KO41、KO16)選択した。KO41細胞株、KO16細胞株ともに野生型に比べて、Gpr81遺伝子発現の有意な減少が認められた。野生型MC3T3-E1細胞と比較して、形態的変化は認めなかった。野生型MC3T3-E1細胞、KO41細胞株、KO16細胞株からゲノム抽出を行った。1.5%アガロースゲルで電気泳動を行い、ともに約1000bpのDNA断片がシングルバンドとして確認した。KO16株ではPAM配列から-7～-4までの4塩基が欠損し、-3に変異が挿入されていた。","subitem_description_type":"Abstract"}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0916-2313","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"前田, 豊信"}],"nameIdentifiers":[{"nameIdentifier":"13531","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"鈴木, 厚子"}],"nameIdentifiers":[{"nameIdentifier":"13532","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"湯澤, 仁"}],"nameIdentifiers":[{"nameIdentifier":"13533","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"馬場, 優"}],"nameIdentifiers":[{"nameIdentifier":"13534","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"加藤, 靖正"}],"nameIdentifiers":[{"nameIdentifier":"13535","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-05-28"}],"displaytype":"detail","filename":"S41(3-4)123-128.pdf","filesize":[{"value":"1.1 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"S41(3-4)123-128.pdf","url":"https://ohu-lib.repo.nii.ac.jp/record/4044/files/S41(3-4)123-128.pdf"},"version_id":"a847c880-da99-4957-9e3e-59a5ce99c388"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"塩基配列; *骨芽細胞; 分散分析; ウエスタンブロッティング; *3T3細胞; ノックアウトマウス; *G-Protein-Coupled Receptors(欠損・欠乏); *CRISPR","subitem_subject_scheme":"Other"},{"subitem_subject":"*GPR81 Protein(欠損・欠乏)","subitem_subject_scheme":"Other"},{"subitem_subject":"マウス; 動物; 歯学","subitem_subject_scheme":"Other"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"CRISPR/Cas9を用いたMC3T3-E1細胞におけるGpr81遺伝子のノックアウト","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"CRISPR/Cas9を用いたMC3T3-E1細胞におけるGpr81遺伝子のノックアウト"}]},"item_type_id":"10002","owner":"3","path":["255"],"pubdate":{"attribute_name":"公開日","attribute_value":"2016-05-28"},"publish_date":"2016-05-28","publish_status":"0","recid":"4044","relation_version_is_last":true,"title":["CRISPR/Cas9を用いたMC3T3-E1細胞におけるGpr81遺伝子のノックアウト"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-05-15T15:00:08.316820+00:00"}