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ラット口蓋粘膜における炭酸ガスレーザーパルス波照射後の治癒過程
https://ohu-lib.repo.nii.ac.jp/records/2786
https://ohu-lib.repo.nii.ac.jp/records/2786f0e3c2a3-b0af-4637-bc88-afaba642cb03
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
KJ00005073478.pdf (2.5 MB)
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| Item type | 紀要論文(ELS)_JAIRO Cloud（WEKO3）対応_596d4a9f(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2008-06-01 | |||||
| タイトル | ||||||
| タイトル | ラット口蓋粘膜における炭酸ガスレーザーパルス波照射後の治癒過程 | |||||
| タイトル | ||||||
| タイトル | Healing Process after Pulsed CO_2 Laser Irradiation on Rat Palatal Mucosa | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | CO_2 laser | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Hsp47 | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | α-SMA | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Factor-VIII | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| ページ属性 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | P(論文) | |||||
| 記事種別(日) | ||||||
| 値 | 原著論文 | |||||
| 記事種別(英) | ||||||
| 言語 | en | |||||
| 値 | Original Article | |||||
| 著者名(日) |
園田, 正人
× 園田, 正人× 山崎, 章 |
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| 著者名よみ |
ソノダ, マサヒト
× ソノダ, マサヒト× ヤマサキ, アキラ |
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| 著者名(英) |
SONODA, Masahito
× SONODA, Masahito× YAMASAKI, Akira |
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| 著者所属(日) | ||||||
| 値 | 奥羽大学大学院歯学研究科顎口腔外科学専攻 | |||||
| 著者所属(日) | ||||||
| 値 | 奥羽大学歯学部口腔病態解析制御学講座口腔病理学 | |||||
| 著者所属(英) | ||||||
| 言語 | en | |||||
| 値 | Ohu University Graduate School of Dentistry, Oral Surgery Major | |||||
| 著者所属(英) | ||||||
| 言語 | en | |||||
| 値 | Department of Oral Medical sciences, Oral Pathology, Ohu University, School of Dentistry | |||||
| 抄録(英) | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Despite the extensive use of the pulsed CO_2 laser, the cellular response and healing process after irradiation with it is not fully understood. We conducted this study to evaluate the healing process of the laser wound by means of histopathology and immunohistochemistry. The CO_2 laser irradiation was made on rat palatal mucosa with the parameters of 600μs pulse duration, 5W peak power, 151.52pps repetition rate, and 14.16J/mm^2 average energy density. The palatal mucosa dissected at 6 hrs and 1, 3, 5, and 7 days after irradiation was fixed and embedded in paraffin. The sections were stained with H-E or Azan-Mallory for histopathological examination or processed immunohistochemically to examine the expression of Hsp47, α-SMA, and Factor-VIII. On day 1 post-irradiation, the laser wound consisted of coagulation necrosis showing the matrix configuration identical to that of the surrounding normal tissue, along the surface of which the emigration of regenerating epithelium was observed. In the tissue surrounding the laser wound, the up-regulation of Hsp47 in fibroblasts and vascular endothelial cells was noted. On day 3, re-epithelization of the wound surface was completed and Hsp47-expressed fibroblasts and vascular endothelial cells progressively infiltrated into the wound. Some fibroblasts exhibited the expression of α-SMA. On day 7, the wound was almost entirely replaced by newly formed fibroblasts and capillaries, most of which appeared to express strong Hsp47. Many of Hsp47-positive fibroblasts in the laser wound also showed α-SMA-expression. The extracellular matrix of the laser wound showed Factor-VIII-positive reaction throughout the experimental period except 6 hours post-irradiation, suggesting the persistence of inflammatory reaction. Since there was no significant difference in the healing process between laser wound and scalpel-made wound, we speculate that laser irradiation and the resultant tissue coagulation may not necessarily disturb the healing process. | |||||
| 雑誌書誌ID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AN1010214X | |||||
| 書誌情報 |
奥羽大学歯学誌 巻 35, 号 2, p. 95-104, 発行日 2008-06 |
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