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苦味に関連する唾液中タンパク質の検出
https://ohu-lib.repo.nii.ac.jp/records/2160
https://ohu-lib.repo.nii.ac.jp/records/216056733e34-2f87-471c-befa-edc5d8bc8d64
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
KJ00000731683.pdf (1.3 MB)
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| Item type | 紀要論文(ELS)_JAIRO Cloud（WEKO3）対応_596d4a9f(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2003-06-30 | |||||
| タイトル | ||||||
| タイトル | 苦味に関連する唾液中タンパク質の検出 | |||||
| タイトル | ||||||
| タイトル | Detection of Proteins Concerning Bitter Taste in Saliva | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | human saliva | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | histatin | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | proline-rich peptide | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | quinine sulfate | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | bitter taste | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| ページ属性 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | P(論文) | |||||
| 記事種別(日) | ||||||
| 値 | 原著論文 | |||||
| 記事種別(英) | ||||||
| 言語 | en | |||||
| 値 | Original Article | |||||
| 著者名(日) |
馬場, 園子
× 馬場, 園子 |
|||||
| 著者名よみ |
ババ, ソノコ
× ババ, ソノコ |
|||||
| 著者名(英) |
BABA, Sonoko
× BABA, Sonoko |
|||||
| 著者所属(日) | ||||||
| 値 | 奥羽大学大学院歯学研究科 | |||||
| 著者所属(英) | ||||||
| 言語 | en | |||||
| 値 | Ohu University Graduate School of Dentistry | |||||
| 抄録(英) | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | To find out salivary proteins related to bitter taste, quinine sulfate showing fluorescence under UV radiation was utilized. Resting human saliva was collected from normal and abnormal subjects for quinine sulfate threshold. The salivary proteins added 0.08% quinine sulfate solution were separated on 2% agarose gel electrophoresis. After detecting the fluorescent band under UV radiation, it was separated with 18.0% SDS-PAGE and then the proteins were transferred to PVDF membrane to elute. These were analyzed with an amino acid sequencer. Comparing the peptides of normal subjects with that of abnormal subjects in SDS-PAGE, a band less than 6.5kDa containing quinine sulfate was recognized on normal subjects. One well-defined band identified with CBB staining of the agarose gel corresponds to the fluorescent portion of the gel, suggesting the quinine might bind to a certain peptide in saliva. Two peptides, Histatin 3, 5, or 6 and basic Proline-Rich Peptide P-E, were detected with partial sequence analysis. These two are candidates as bitter-binding peptides, although their functions have not yet been identified. | |||||
| 雑誌書誌ID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AN1010214X | |||||
| 書誌情報 |
奥羽大学歯学誌 巻 30, 号 2, p. 189-196, 発行日 2003-06-30 |
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